| Suppression of IL-1beta-induced MUC5AC Gene Expression by Ginkgo biloba Extract(EGb 761) in Human Airway Epithelial Cells |
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Jung Hyun Chang, Jeong Hong Kim, Kun Wayn Lee, Chang Il Cho, Ju Hyun Chun, Kyung Su Kim |
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
2The Airway Mucus Research Institute, Yonsei University College of Medicine, Seoul, Korea.
3Department of Otorhinolaryngology, Cheju University College of Medicine, Jeju, Korea. |
| 은행잎 추출물(EGb 761)에 의한 IL-1β 유도 MUC5AC 유전자 발현의 억제 |
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장정현, 김정홍, 이근완, 조창일, 전주현, 김경수 |
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연세대학교 의과대학 이비인후과학교실,1 기도점액연구소,2 제주대학교 의과대학 이비인후과학교실3 |
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| Abstract |
Background and Objectives
The purpose of this study is to investigate whether Ginkgo biloba extract (EGb 761) can suppress IL-1beta-induced MUC5AC gene expression in NCI-H292 human airway epithelial cells and to discover what its possible mechanism is.
MATERIALS AND METHODS
NCI-H292 human pulmonary mucoepidermoid carcinoma cell line was used. MUC5AC mRNA and protein were measured using the RT-PCR and Western blot analysis. The activation of mitogen activated protein kinases (MAPKs) were determined by means of the Western blot analysis.
Results
MUC5AC was induced by treating the NCI-H292 cells with 10 ng/ml of IL-1beta for 24 hours. A pre-treatment of 200 microgram/ml of EGb 761 significantly suppressed the IL-1beta induced MUC5AC expression. The inhibition of MUC5AC gene expression by EGb 761 was noted to be suppressed via both the extracellular signal-regulated kinase (ERK) and the p38 MAPK pathways in a kinase-specific inhibitor study.
Conclusion
EGb 761 suppresses IL-1beta-induced MUC5AC gene expression in the human airway epithelial cells. Therefore, it may be considered as a possible anti-hypersecretory agent. |
| Key Words:
Ginkgo biloba extract 761;Interleukin-1beta;Mucins;Epithelial cells |
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