Journal of Rhinology 2007;14(1):49-55.
Published online May 31, 2007.
Suppression of IL-1beta-induced MUC5AC Gene Expression by Ginkgo biloba Extract(EGb 761) in Human Airway Epithelial Cells
Jung Hyun Chang, Jeong Hong Kim, Kun Wayn Lee, Chang Il Cho, Ju Hyun Chun, Kyung Su Kim
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
2The Airway Mucus Research Institute, Yonsei University College of Medicine, Seoul, Korea.
3Department of Otorhinolaryngology, Cheju University College of Medicine, Jeju, Korea.
은행잎 추출물(EGb 761)에 의한 IL-1β 유도 MUC5AC 유전자 발현의 억제
장정현, 김정홍, 이근완, 조창일, 전주현, 김경수
연세대학교 의과대학 이비인후과학교실,1 기도점액연구소,2 제주대학교 의과대학 이비인후과학교실3
Abstract
Background and Objectives
The purpose of this study is to investigate whether Ginkgo biloba extract (EGb 761) can suppress IL-1beta-induced MUC5AC gene expression in NCI-H292 human airway epithelial cells and to discover what its possible mechanism is.
Materials and Method
S: NCI-H292 human pulmonary mucoepidermoid carcinoma cell line was used. MUC5AC mRNA and protein were measured using the RT-PCR and Western blot analysis. The activation of mitogen activated protein kinases (MAPKs) were determined by means of the Western blot analysis.
Results
MUC5AC was induced by treating the NCI-H292 cells with 10 ng/ml of IL-1beta for 24 hours. A pre-treatment of 200 microgram/ml of EGb 761 significantly suppressed the IL-1beta induced MUC5AC expression. The inhibition of MUC5AC gene expression by EGb 761 was noted to be suppressed via both the extracellular signal-regulated kinase (ERK) and the p38 MAPK pathways in a kinase-specific inhibitor study.
Conclusion
EGb 761 suppresses IL-1beta-induced MUC5AC gene expression in the human airway epithelial cells. Therefore, it may be considered as a possible anti-hypersecretory agent.
Key Words: Ginkgo biloba extract 761;Interleukin-1beta;Mucins;Epithelial cells




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