| Effect of Macrolides on the Interleukin-1beta-mediated MUC2/5AC Genes Expression and Mucin Secretion in Human Airway Epithelial Cells |
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Yong Dae Kim, Jung Suck Cho, Hak Soo Kim, Si Youn Song |
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Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr |
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| Abstract |
Background and Objectives
Macrolide is a relatively effective drug in chronic bronchiolitis and chronic sinusitis with mucous hypersecretion. However, the anti-secretory effect of macrolide is not clear. The aim of this study was to evaluate the effect of macrolide on the interleukin -1beta (IL-1beta)-induced MUC2/5AC genes expression and mucin secretion.
Matrials and Methods: We observed effects of roxithromycin and clarithromycin on the IL-1beta-induced MUC2/5AC genes expression and mucin secretion in cultured human airway NCI-H292 epithelial cells. The steady state mRNA levels of MUC2/5AC and mucin secretion were determined by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay.
Results
Roxithromycin attenuated the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion. When roxithromycin treated before exposure to IL-1beta and after exposure to IL-1beta in cultured cells, both treatment methods inhibited the IL-1beta-induced MUC2/5AC genes expression and mucin secretion. However, clarithromycin did not suppress the IL-1beta-mediated MUC2/5AC genes expression and mucin secretion.
Conclusion
This result suggests that roxithromycin inhibits the IL-1beta-mediated mucin secretion through inhibition of MUC2/5AC genes expression. |
| Key Words:
MUC2;MUC5AC;Mucin;Roxithromycin;Clarithromycin |
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