J Rhinol > Volume 23(1); 2016
Journal of Rhinology 2016;23(1):17-23.
Published online May 31, 2016.
DOI: https://doi.org/10.18787/jr.2016.23.1.17   
Effect of High-Insulin on MUC4, MUC5AC, and MUC5B Expression in Airway Epithelial Cells
Hyung Gyun Na, Chang Hoon Bae, Yoon Seok Choi, Si Youn Song, Hyun Jung Jin, Yong Dae Kim
1Department of Otorhinolaryngology-Head and Neck Surgery, College of Medicine, Yeungnam University, Daegu, Korea. ydkim@med.yu.ac.kr
2Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.
3Regional Center for Respiratory Diseases, Yeungnam University Medical Center, Daegu, Korea.
인체 호흡기 상피세포에서 고농도 인슐린에 의한 MUC4, MUC5AC, MUC5B 점액 유전자의 발현
나형균, 배창훈, 최윤석, 송시연, 진현정, 김용대
영남대학교 의과대학 이비인후-두경부외과학교실,1 내과학교실,2 영남대학교병원 권역 호흡기 전문질환센터3
Abstract
Background and Objectives
Insulin is a peptide hormone that regulates the metabolism of carbohydrates and fats by promoting the absorption of glucose from the blood to skeletal muscles. Insulin has been reported to be closely related to cardiovascular, respiratory, and endocrine disease. However, the effect of insulin on production of major mucins in human airway epithelial cells has not been reported. Therefore, this study investigated the relationship between high levels of insulin and mucin in human airway epithelial cells.
Materials and Method
S: This study analyzed the effect of high level of insulin on MUC4, MUC5AC, and MUC5B expression using reverse transcription-polymerase chain reaction and enzyme-linked immunosorbent assay in human airway epithelial cells.
Results
In human NCI-H292 airway epithelial cells, high level of insulin significant increased MUC4, MUC5AC, and MUC5B mRNA expression and glycoprotein production. In the primary cultures of normal nasal epithelial cells, high level of insulin also increased MUC4, MUC5AC, and MUC5B expression.
Conclusion
These results suggest that insulin plays a role in control of mucus hypersecretion in human airway epithelial cells.
Key Words: Insulin;MUC4;MUC5AC;MUC5B;Human airway epithelial cell




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