Local Allergic Inflammation in Chronic Rhinosinusitis With Nasal Polyps Could Influence on Disease Severity and Olfaction

Byung kee Youn; Dong-Kyu Kim; Byung Hum Kim; Hyung Gu Kim; Jin Hyeok Jeong; Seok Hyun Cho

doi:10.18787/jr.2021.00363

J Rhinol > Volume 28(3); 2021

Youn, Kim, Kim, Kim, Jeong, and Cho: Local Allergic Inflammation in Chronic Rhinosinusitis With Nasal Polyps Could Influence on Disease Severity and Olfaction

Original Article

Journal of Rhinology 2021;28(3):147-152.

Published online: November 26, 2021

DOI: https://doi.org/10.18787/jr.2021.00363

Local Allergic Inflammation in Chronic Rhinosinusitis With Nasal Polyps Could Influence on Disease Severity and Olfaction

Byung kee Youn, MD^1,^*, Dong-Kyu Kim, MD^2,^*, Byung Hum Kim, MD¹, Hyung Gu Kim, MD¹, Jin Hyeok Jeong, MD¹, Seok Hyun Cho, MD¹

¹Department of Otorhinolaryngology-Head and Neck Surgery, Hanyang University College of Medicine, Seoul, Republic of Korea

²Department of Otorhinolaryngology-Head and Neck Surgery, Chuncheon Sacred Heart Hospital, Hallym University College of Medicine, Chuncheon, Republic of Korea

Address for correspondence: Seok Hyun Cho, MD, Department of Otorhinolaryngology-Head and Neck Surgery, Hanyang University College of Medicine, 222-1 Wangshimni-ro, Seongdong-gu, Seoul 04763, Republic of Korea
Tel: +82-2-2290-8583, Fax: +82-2-2-2290-8588, E-mail: shcho@hanyang.ac.kr

*These authors contributed equally to this work.

Received May 21, 2021 Revised June 28, 2021 Accepted July 1, 2021

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background and Objectives

Chronic rhinosinusitis with nasal polyps (CRSwNP) is a multifactorial disease resulting from inflammation of the nasal cavity and paranasal sinuses. Systemic allergic inflammation is an important cause of CRSwNP; however, the effect of local allergic inflammation is unclear. This study was designed to investigate the effect of local allergic inflammation in CRSwNP.

Materials and Methods

The study included 11 patients with CRSwNP and 18 control subjects. Olfactory function was measured with the Korean Version of Sniffin’s stick test. Nasal lavage fluids (NLFs) were collected from all subjects and analyzed for total IgE, eosinophilic cationic protein (ECP), and cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-4, IL-10, IL-17A, interferon-γ). Flow cytometry was used to measure various inflammatory cells in the NAL fluids.

Results

On analysis of flow cytometry and enzyme-linked immunosorbent assay, we found that CRSwNP patients had significantly increased eosinophil (%) and ECP levels in NLFs. In addition, there was significant local-systemic correlation between ECP level in NLFs and blood eosinophils (%) (r=0.391); however, there was no significant association between eosinophils (%) in NLFs and blood eosinophils. Moreover, in CRSwNP patients, the severity of disease was related with blood eosinophil (%), eosinophil (%), and ECP levels in NLFs, whereas olfactory function was associated with blood eosinophil (%) and ECP levels in NLFs.

Conclusion

CRSwNP is a disease with high allergic inflammation that has negative impacts on the severity of disease and olfactory function. Therefore, we suggest that control of local allergic inflammation will be helpful to treat CRSwNP patients.

KEY WORDS: Chronic rhinosinusitis; Polyp; Local; Immune

INTRODUCTION

Chronic rhinosinusitis (CRS) is defined as an inflammatory condition involving the paranasal sinuses, which persists for 12 weeks or longer. It is associated with two or more symptoms of either nasal obstruction or nasal discharge (anterior/posterior nasal drip), facial pain/pressure, and/or reduction/loss of smell. Currently, this disease classified as subgroups of patients based on nasal endoscopic findings, either accompanied by nasal polyps (CRSwNP) or without nasal polyps (CRSsNP) [1]. Generally, CRSsNP comprises more than two-thirds of cases and is less likely to be managed by surgical intervention, whereas CRSwNP represents 20%-25% of cases and frequently need surgical intervention [2]. The major pathogenesis of CRS is considered as alterations in mucociliary clearance, abnormalities in the sinonasal epithelial cell barrier, and tissue remodeling [2-4]. Additionally, the upregulated host innate and adaptive immune responses may be involved in its pathogenesis [2-4]. Specifically, most patients with CRSwNP have shown a predominant type 2 immune profile, compare to those with CRSsNP [5]. However, the exact pathogenesis of CRSwNP is still unclear.

Recently, the presence of local allergy in the nasal cavity was found in a number of patients diagnosed previously with non-allergic rhinitis [6-8]. Evidences for local allergy are supported by clinical symptoms, local production of allergen-specific immunoglobulin E (sIgE), a type 2 helper T cell inflammatory pattern in nasal secretions during natural exposure to aeroallergens, and a positive response to nasal allergen provocation with local nasal production of slgE to aeroallergens, tryptase, and eosinophil cationic protein (ECP) [9]. However, to date, there was little known about the effect of local immune response on clinical relevance in patients with CRSwNP. Therefore, in the present study, we evaluated the local immunologic profile using nasal lavage fluids (NLFs) in CRSwNP patients and also investigated its clinical relevance, such as disease severity and olfactory function.

MATERIALS AND METHODS

Subjects and sampling of nasal fluids

From December 2015 to August 2016, we enrolled 29 consecutive patients. The diagnosis of CRS was based on personal medical history, physical examination, nasal endoscopy and CT findings of the nasal cavity with sinuses according to the 2012 European position paper on rhinosinusitis and nasal polyps (EPOS) guidelines [1]. Patient exclusion criteria were as follows: 1) younger than 18 years old; 2) previous treatment with antibiotics, systemic or topical corticosteroids, or other immune-modulating drugs up to 4 weeks before surgery; and 3) conditions such as unilateral rhinosinusitis, antrochoanal polyps, allergic fungal sinusitis, cystic fibrosis, or immotile ciliary disease. Samples from control subjects were obtained from patients with septal surgeries who do not have any sinonasal diseases. Paranasal CT images were staged with the LundMackay score for identifying CRS severity. Allergic rhinitis was diagnosed with a medical history and skin prick test. The diagnosis of asthma was based on the medical history and lung function analysis, including methacholine challenge test by pulmonologists. Patients were asked to complete the questionnaire reporting their symptoms (itching, sneezing, rhinorrhea, and nasal obstruction) at baseline and we calculated total nasal symptom score (TNSS). Quality of life (QOL) was measured on a 10-point visual analog scale. Additionally, all subjects underwent psychological olfactory test using the Korean version II of the Sniffin’ Sticks Test and calculated the sum of Threshold-Discrimination-Identification (TDI). Moreover, we collected NLFs as previous described method under general anesthesia [10]. Briefly, under general anesthesia, we flushed each nostril with 5 mL of isotonic saline. The fluid was aspirated with a suction collector and then, immediately transferred to the laboratory bench. After the removal of crust and debris, the fluids were centrifuged at 6,000 rpm for 5 min. After centrifuges, the cell pellets were used for the flow cytometry and the supernatants were analyzed by ImmunoCAP and enzyme-linked immunosorbent assay (ELISA). This study was approved by the Institutional Review Board of Hanyang University Medical Center with a waiver of informed consent (IRB No. HYI 12-012).

Flow cytometry

We used the same antibody panel and gating strategy for the detection of nasal T cells (CD3), B cell (CD19), macrophages (CD14), dendritic cells (CD11c), and eosinophils (CD16) as previously described [10]. Flow cytometry was performed using a FACS Canto II (BD Biosciences, San Jose, CA, USA) and data were analyzed using Flow Jo ver. 9.3.2 (Treestar, Inc, San Carlos, CA, USA).

ImmunoCAP and ELISA

The concentrations of total IgE was measured using the ImmunoCAP^® assay (Phadia, Uppsala, Sweden). Immunologic profiled (ECP, tumor necrosis factor [TNF]-α, interleukin [IL]-4, IL-10, IL-17A, and interferon [IFN]-γ) were also analyzed using commercially available ELISA kits according to the manufacture’s recommendations.

Statistical analysis

Data were presented as medians with/without interquartile range and statistical significance (p<0.05) was analyzed using the Mann-Whitney U test. The relationships between two variables were examined using Spearman correlation analysis. All statistical analyses were performed using SPSS Statistics 21.0 (IBM Corp., Armonk, NY, USA).

RESULTS

Clinical characteristics of subjects

The clinical characteristics of each study group are summarized in Table 1. There was no difference in sex, allergic rhinitis, asthma, TNSS, QOL, and total IgE between the CRSwNP and controls. However, CRSwNP group showed significantly increased age, higher CT score, and eosinophils (%) compared to control, whereas TDI score was lower in CRSwNP than in control.

Local cellular and humoral immunologic profile in chronic rhinosinusitis with nasal polyp

To evaluate the difference in local cellular response between two groups, we performed flow cytometry using NLFs. We found that there was no difference in innate immune cells (macrophages, dendritic cells, and neutrophils) and lymphocytes (T cells and B cells) between control and CRSwNP. However, CRSwNP group had significantly increased local eosinophilia compared to control (p=0.021) (Table 2).

Next line, to investigate the difference of local humoral response between two groups, we evaluated the immunologic profile of NLFs using immunoCAP and ELISA. There was no significant difference in Th1 (TNF-α, IFN-γ), Th2 (IL-4, total IgE), Th17 (IL-17A), and Treg (IL-10) markers between two groups. However, the expression of ECP level was significantly increased in CRSwNP group, compared to control (p=0.037) (Table 3).

Effect of local allergic inflammation on the clinical relevance of patients with chronic rhinosinusitis with nasal polyp

On analysis of the correlation between local and systemic allergic inflammation, we found a significant local-systemic correlation between ECP level in NLFs and blood eosinophil (%) (r=0.391) (Fig. 1). However, there was no significant association for eosinophil (%) in NLFs with blood eosinophil (%) or ECP level in NLFs.

In addition, we assessed the clinical relevance of local or systemic allergic inflammation, such as disease severity and olfactory function (Fig. 2). We observed a significant correlation of blood eosinophil with disease severity and olfactory function, whereas eosinophil (%) in NLFs showed an only significant relationship on disease severity, not olfactory function. However, ECP level in NLFs showed a significant correlation with disease severity and olfactory function (r=0.387 and r=-0.415, respectively).

DISCUSSION

Numerous studies have supported that systemic allergic inflammation may be an important cause of development of CRSwNP [2,3,11,12]. However, studies for local allergic inflammation on CRSwNP are still limited [13-15]. Thus, in the present study, we evaluated the local allergic inflammation status and analyzed its clinical relevance in patients with CRSwNP. Our major findings can be summarized as follows: 1) CRSwNP patients showed an increased local allergic inflammation, such as eosinophils (%) and ECP level, compared to controls. 2) Among local allergic inflammation, ECP level in nasal secretion was a significantly associated with blood eosinophil (%), but there was no relationship between eosinophil (%) in blood and nasal secretion. 3) Interestingly, we found that disease severity and olfactory function in patients with CRSwNP were well reflected by blood eosinophil (%) and ECP level in nasal secretion.

Eosinophils are responsible for tissue damage and inflammation in many diseases, specifically in allergic disease. It is known that several mediators derived from eosinophils can damage epithelial cells, stimulate epithelial-to-mesenchymal transition, and alter mechanical responses of airways [16-19]. To date, correlations between disease severity and eosinophils measured in blood or nasal tissues are well established [15,20-23]. These studies described that eosinophilia correlates strongly with type 2 immune and inflammatory responses, and eosinophilic CRSwNP patients showed a higher prevalence and severity of asthma. Additionally, one recent large scale study [24], the Japanese Epidemiological Survey of Refractory Eosinophilic Chronic Rhinosinusitis study which evaluated eosinophils in the blood and tissue, suggested that blood eosinophils, ethmoid disease, asthma, and aspirin intolerance are well associated with the recurrence rate of CRS patients. Consistent with these reports, eosinophilia in blood and nasal secretion was significantly associated with disease severity in this study, and we also observed the positive relationship between ECP level in nasal secretion and disease severity. Moreover, several studies demonstrated that olfactory dysfunction in CRS patients correlates local eosinophilia [25,26]. Although we could not find a significant relationship between eosinophilia in nasal secretion and olfactory function, ECP level in nasal secretion had negatively correlated with olfactory function. Together, these data indicated that ECP level in nasal secretion as a marker for local allergic inflammation is more well-reflected the clinical relevance in patients with CRSwNP, compare to eosinophilia in nasal secretion.

However, our study has some limitations. First, in this study, we could not evaluate tissue eosinophilia, because tissue biopsies were not performed. Second, CRSwNP generally has two endotypes, such as eosinophilic and non-eosinophilic types. Eosinophilic CRSwNP is characterized by increased type 2 immune response with tissue eosinophilia, whereas non-eosinophilic CRSwNP has a mixed immune response with predominant tissue infiltration of neutrophils and lymphocytes. Thus, the effect of local allergic inflammation may be different between each endotypes, but we could not differentiate the endotypes due to lack of nasal tissues obtained from patients. Third, this study showed a different mean age between the two groups. This difference may affect our outcomes as a confounding factor because the immune system undergoes age-related changes, including a gradual increase in the production and circulation of proinflammatory cytokines.

In conclusion, the elevated local allergic inflammation is detected in patients with CRSwNP. Eosinophilia and increased ECP level in nasal secretion are a signature marker for local allergic inflammation in CRSwNP patients. In addition, Eosinophilia and ECP level in nasal secretion is correlated with disease severity. Moreover, olfactory dysfunction correlates with ECP level in nasal secretion. Therefore, we suggest that clinicians have an interest in the control of local allergic inflammation when those treat CRSwNP patients.

Notes

Conflicts of Interest

The authors have no potential conflicts of interest to disclose.

Author Contributions

Conceptualization: Byung kee Youn, Seok Hyun Cho. Data curation: Byung kee Youn, Byung Hum Kim, Seok Hyun Cho. Formal analysis: Byung kee Youn, Byung Hum Kim. Funding acquisition: Seok Hyun Cho. Investigation: Byung kee Youn, Dong-Kyu Kim. Methodology: Byung kee Youn, Seok Hyun Cho. Project administration: Byung kee Youn. Resources: Byung kee Youn, Dong-Kyu Kim. Software: Dong-Kyu Kim. Supervision: Dong-Kyu Kim, Jin Hyeok Jeong. Validation: Hyung Gu Kim, Jin Hyeok Jeong. Visualization: Byung kee Youn, Hyung Gu Kim, Seok Hyun Cho. Writing—original draft: Dong-Kyu Kim, Seok Hyun Cho. Writing—review & editing: Dong-Kyu Kim.

Funding Statement

This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP; Ministry of Science, ICT & Future Planning) (No. 2017R1D1A1B03028797) and the research fund of Hanyang University (HY-2015).

Fig. 1.

Evaluation of interaction local to systemic allergic inflammation (*p<0.05). A: Relationship between blood and nasal eosinophils (%). B: Realtionship between nasal ECP and eosinophils (%). ECP, eosinophil cationic protein.

Fig. 2.

Effect of local allergic inflammation on clinical relevance. A: Relationship between blood eosinophils (%) and CT score/olfactory function (TDI). B: Relationship between nasal eosinophils (%) and CT score/olfactory function (TDI). C: Relationship between nasal ECP level and CT score/olfactory function (TDI). *p<0.05. TDI, Threshold-Discrimination-Identification. ECP, eosinophil cationic protein.

Table 1.

Clinical characteristic of the subjects

Characteristic	Control (n=18)	CRSwNP (n=11)	p value
Sex (M:F)	15:3	9:2	0.644
Age (year)	23.5 (19.8–33.5)	49.0 (26.0–66.0)	0.035^*
Allergic rhinitis (%)	66.7	45.5	0.230
Asthma (%)	0	9.1	0.379
TNSS	7.0 (5.5–9.0)	5.0 (4.0–8.0)	0.191
QOL	4.0 (2.0–4.0)	3.0 (1.0–4.0)	0.578
TDI	36.0 (32.5–36.5)	27.0 (12.0–32.0)	0.002^*
CT score	0.0 (0.0–0.0)	15.0 (12.5–16.0)	0.001^**
Total IgE	108.2 (39.2–332.8)	130.7 (117.4–200.0)	0.643
Eosinophils (%)	2.6 (1.2–3.9)	4.5 (2.7–6.2)	0.039^*

^* p<0.05;

^** p<0.01.

CRSwNP, chronic rhinosinusitis with nasal polyps; TNSS, total nasal symptom score; QOL, quality of life; TDI, Threshold-Discrimination-Identification; IgE, immunoglobulin E

Table 2.

Comparison of local cellular immune responses between control and chronic rhinosinusitis with nasal polyps (CRSwNP)

Variable	Control	CRSwNP	p value
WBC count (/mm³)	14,160 (8,349–22,925)	100,199 (10,960–215,799)	0.084
Macrophages (%)	0.55 (0.31–1.61)	1.20 (0.38–1.54)	0.492
Dendritic cells (%)	1.02 (0.42–1.34)	0.28 (0.21–0.53)	0.076
T cells (%)	18.86 (5.38–34.10)	5.83 (3.97–12.54)	0.102
B cells (%)	4.75 (1.17–8.89)	1.87 (0.53–3.50)	0.076
Neutrophils (%)	67.08 (32.28–70.45)	44.54 (26.31–60.61)	0.298
Eosinophils (%)	3.53 (1.98–10.03)	10.28 (7.87–24.71)	0.021^*

^* p<0.05

Table 3.

Comparison of local humoral immune responses between control and chronic rhinosinusitis with nasal polyps (CRSwNP)

Variable	Control	CRSwNP	p value
ECP (μg/L)	18.9 (13.5–65.7)	71.2 (42.1–143.8)	0.037^*
TNF-α (pg/mL)	1.5 (0.0–4.1)	4.3 (2.5–9.9)	0.176
IL-4 (pg/mL)	2.4 (0.0–6.3)	0.0 (0.0–5.7)	0.275
IL-10 (pg/mL)	0.0 (0.0–0.0)	0.0 (0.0–5.1)	0.298
IL-17A (pg/mL)	0.0 (0.0–0.0)	0.0 (0.0–1.21)	0.940
IFN-γ (pg/mL)	8.4 (2.1–15.7)	27.5 (1.1–32.9)	0.433
Total IgE (kU/L)	5.5 (4.7–9.7)	5.3 (4.0–9.4)	0.774

^* p<0.05.

ECP, eosinophilic cationic protein; IL, interleukin; IFN, interferon; IgE, immunoglobulin E

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