Journal of Rhinology 2005;12(1):16-20.
Published online May 31, 2005.
Repeated Ozone Exposure Induces Th2 Immune Responses in Mice
Yong De Jin, Young Kyu Park, Yong Min Kim, Seok Won Park, Ki Sang Rha, Chan Il Park
1Department of Otolaryngology, College of Medicine, Yanbian University, Yanji, China.
2Department of Otolaryngology, College of Medicine, Chungnam National University, Daejeon, Korea.
마우스에서 반복적인 오존노출에 의한 Th-2 면역반응의 유발
김영덕, 박영규, 김용민, 박석원, 나기상, 박찬일
연변대학교 의과대학 이비인후과학교실,1 충남대학교 의과대학 이비인후과학교실2
Background and Objectives
It has been reported that ozone exposure exacerbate allergic rhinitis symptoms and may contribute to increase allergic rhinitis prevalence. However, a causal relationship still remained unsolved. The purpose of this study is to investigate whether prolonged exposure to ozone induce Th2 immune response without allergen.
Fourteen BALB/c mice were divided into two groups: control group and ozone exposure group. Mice were exposed to 0.3 ppm of ozone for 4 hours a day, 3 times per week, for 4 weeks. At 24 hour after the last ozone exposure, nasal lavage fluid (NLF) was obtained to measure the levels of cytokine IL-4, IL-5, and IFN-gamma. After lavage fluid was obtained, blood was obtained via inferior vena cava to measure the amount of total IgE and IgG1. The concentration of cytokines and immunoglobulins was measured using the ELISA method. In addition, Luna staining was performed to identify eosinophils infiltrated in nasal mucosa.
The levels of IL-4 and IL-5 in NLF were significantly increased in ozone exposure group compared with control group. But the level of IFN-gamma in NLF shows no significant difference between two groups. Serum total IgE and IgG1 were significantly increased in ozone exposure group compared with control group. On histologic examination, number of eosinophils infiltrating nasal mucosa was significantly increased in ozone exposure group.
These results suggest that repeated ozone exposure induces Th2 response in the nasal mucosa of mice.
Key Words: Ozone;Th2 response;Immunoglobulin;Cytokine;Mouse

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