Journal of Rhinology 2005;12(1):32-38.
Published online May 31, 2005.
The Inhibitory Effect of Anti-P-Selectin, Anti-PSGL-1 and Anti-CD49d Antibodies on Experimental Allergic Airway Inflammation in Mice
Seok Won Park, A Young Kim, Yong De Jin, Bon Seok Koo, Yong Min Kim, Yong Ho Park, Chan Il Park, Ki Sang Rha
1Department of Otolaryngology, College of Medicine, Chungnam National University, Daejeon, Korea. ksrha@cnu.ac.kr
2Department of Otolaryngology, College of Medicine, Yanbian University, Yanji, China.
실험적으로 기도 알레르기를 유발시킨 마우스에 투여한 항 P-Selectin, 항 PSGL-1, 항 CD49d 항체의 염증억제효과
박석원, 김아영, 김영덕, 구본석, 김용민, 박용호, 박찬일, 나기상
충남대학교 의과대학 이비인후과학교실,1 연변대학교 의학원 이비인후과학교실2
Abstract
Background and Objectives
Eosinophil recruitment in allergic inflammation is dependent on the interactions between adhesion molecules. The objective of this study was to determine whether blocking of P-selectin glycoprotein ligand-1 (PSGL-1), P-selectin or CD49d adhesion molecules using monoclonal antibodies can reduce eosinophil recruitment in mice with the airway allergy.
MATERIALS AND METHODS
Seven different groups of mice were used. One experimental group of mice (group A) served as naive control and sensitized with phosphate-buffered saline (PBS). Six experimental groups of mice (groups B to G) were sensitized to ovalbumin (OVA). The mice in group B were treated with PBS before OVA aerosol challenge and served as positive controls. The mice in other remaining groups were treated with monoclonal antibodies (mAb) against specific adhesion molecules before OVA challenge; anti-P-selectin mAb (group C), anti-PSGL-1 mAb (group D), anti-CD49d mAb (group E), anti-P-selectin mAb & anti-CD49d mAb (group F), anti PSGL-1 mAb & anti-CD49d mAb (group G). Eosinophils in the nasal mucosa and bronchoalveolar lavage (BAL) fluid in each animal group were measured.
Results
Mucosal eosinophilic infiltrations were significantly reduced at the mice in the group C, F or G compared with group B. Eosinophils in BAL fluid were significantly reduced only at the group C mice.
Conclusion
These results suggest that blockade of P-selectin is superior to blockade of PSGL-1 or CD49d in the inhibitory effect against eosinophil recruitment.
Key Words: Allergy;Animal experimentation;Cell adhesion molecules;P-selectin;Eosinophils


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