Journal of Rhinology 2005;12(2):92-96.
Published online November 30, 2005.
15-Lipoxygenase-1 Mediates Mucociliary Differentiation in Normal Human Nasal Epithelial Cells
Jung Hyun Chang, Jeong Hong Kim, Joo Heon Yoon, Jeung Gweon Lee, Hyung Seok Seo, Kyung Su Kim
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea.
2The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Korea.
3Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea.
사람 정상 코점막 상피세포에서 15-Lipoxygenase-1에 의한 점액섬모 분화
장정현, 김정홍, 윤주헌, 이정권, 서형석, 김경수
연세대학교 의과대학 이비인후과학교실,1 기도 점액연구소,2 두뇌한국 21 의과사업단3
Background and Objectives
15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here we investigated the relation between 15-LO-1 expression and the differentiation of normal human nasal epithelial (NHNE) cells.
NHNE cells, RT-PCR, Western blot analysis, and scanning electron microscopy (SEM) were used.
In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in NHNE cells increased time-dependently, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 time-dependently induced 15-LO-1 expression at a concentration of 1 ng/mL. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In SEM, the ciliated epithelium was observed with the treatment of IL-4.
Our findings suggest that 15-LO-1 may be related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of NHNE cells.
Key Words: Arachidonate, 15-Lipoxygenase;Nasal mucosa;Cell differentiation;Cilia

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