| 15-Lipoxygenase-1 Mediates Mucociliary Differentiation in Normal Human Nasal Epithelial Cells |
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Jung Hyun Chang, Jeong Hong Kim, Joo Heon Yoon, Jeung Gweon Lee, Hyung Seok Seo, Kyung Su Kim |
1Department of Otorhinolaryngology, Yonsei University College of Medicine, Seoul, Korea. ydrhinol@yumc.yonsei.ac.kr
2The Airway Mucus Institute, Yonsei University College of Medicine, Seoul, Korea.
3Brain Korea 21 Project for Medical Science, Yonsei University College of Medicine, Seoul, Korea. |
| 사람 정상 코점막 상피세포에서 15-Lipoxygenase-1에 의한 점액섬모 분화 |
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장정현, 김정홍, 윤주헌, 이정권, 서형석, 김경수 |
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연세대학교 의과대학 이비인후과학교실,1 기도 점액연구소,2 두뇌한국 21 의과사업단3 |
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| Abstract |
Background and Objectives
15-lipoxygenase-1 (15-LO-1) is involved in the differentiation of human tracheobronchial epithelial cells. Here we investigated the relation between 15-LO-1 expression and the differentiation of normal human nasal epithelial (NHNE) cells.
MATERIALS AND METHODS
NHNE cells, RT-PCR, Western blot analysis, and scanning electron microscopy (SEM) were used.
Results
In retinoic acid (RA)-sufficient culture media, 15-LO-1 expression in NHNE cells increased time-dependently, but its expression was undetectable in RA-deficient culture media. Moreover, in RA-deficient culture media, IL-4 time-dependently induced 15-LO-1 expression at a concentration of 1 ng/mL. In addition, MUC8 gene expression, a marker of mucociliary differentiation, was up-regulated by 15-LO-1, which was itself induced by IL-4. In SEM, the ciliated epithelium was observed with the treatment of IL-4.
Conclusion
Our findings suggest that 15-LO-1 may be related to the differentiation of human nasal epithelium, and that it may mediate the mucociliary differentiation of NHNE cells. |
| Key Words:
Arachidonate, 15-Lipoxygenase;Nasal mucosa;Cell differentiation;Cilia |
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