Effects of Corticosteroid on Expression of IL-18 in the Airway Mucosa of Allergic Rhinitis Mouse Model

Kim, Si Whan; Jeon, Yoon Kyung; Hong, Seok Chan; Won, Tae Bin; Kim, Yong Min; Rhee, Chae Seo; Min, Yang Gi

Original Article

Journal of Rhinology 2006;13(2):101-106.
Published online November 30, 2006.

Effects of Corticosteroid on Expression of IL-18 in the Airway Mucosa of Allergic Rhinitis Mouse Model

Si Whan Kim, Yoon Kyung Jeon, Seok Chan Hong, Tae Bin Won, Yong Min Kim, Chae Seo Rhee, Yang Gi Min

¹Department of Otorhinolaryngology-Head and Neck Surgery, Seoul National University College of Medicine, Seoul, Korea. ygmin312@dreamwiz.com
²Department of Pathology, Seoul National University College of Medicine, Seoul, Korea.
³Department of Otorhinolaryngology-Head and Neck Surgery, Konkuk University School of Medicine, Seoul, Korea.

기도 알레르기 생쥐모델에서의 IL-18 발현에 대한 스테로이드의 효과

김시환, 전윤경, 홍석찬, 원태빈, 김용민, 이재서, 민양기

서울대학교 의과대학 이비인후과학교실,¹ 병리학교실,² 건국대학교 의과대학 이비인후과학교실³

Abstract

Background: This study aimed to investigate the release and response of IL-18 to steroid treatment in the allergic rhinitis (AR) mouse model.
MATERIALS AND METHODS
BALB/c mice were sensitized systematically using an intraperitoneal ovalbumin (OVA) injection and locally by OVA inhalation. The steroid treatment group had an intraperitoneal dexamethasone injection. Symptom scores, eosinophil counts in nasal septal mucosa, and IL-18 concentrations in nasal and lung lavage fluid were analyzed.
Results
The symptom scores and eosinophil counts of the negative control and steroid treatment groups were significantly lower than those of the positive control group (p<.01). Meanwhile IL-18 concentrations of nasal lavage fluid of the three groups were not significantly different (56.68+/-9.57, 63.39+/-8.93, and 64.47+/-6.83 pg/mL, repectively). IL-18 concentration of lung lavage fluid was significantly different between the positive control and steroid treatment groups (430.75+/-154.54 and 69.94+/-14.26pg/mL respectively, p=.028).
Conclusion
In this study, IL-18 concentration increased not in the nasal lavage fluid but in the lung lavage fluid in AR mouse model. The increased IL-18 concentration decreased after the steroid therapy. This result suggests that the role of IL-18 in the pathogenesis of AR may be different from that of asthma.

Key Words: Allergic rhinitis;Interleukin-18;Glucocorticoid;Corticosteroid