| Airway Remodeling in Mice with Experimentally Induced Airway Allergy |
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Hyo Seon Kim, Dong Hyun Kim, Jae Yong Park, Jin Woong Choi, Yong Min Kim, Ki Sang Rha |
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Department of Otolaryngology-Head and Neck Surgery, Chungnam National University College of Medicine, Daejeon, Korea. ksrha@cnu.ac.kr |
| 기도 알레르기 마우스 모델에서 기도재형성에 관한 연구 |
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김효선, 김동현, 박재용, 최진웅, 김용민, 나기상 |
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충남대학교 의과대학 이비인후과학교실, 암공동연구소 |
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| Abstract |
Background and Objectives
Allergic rhinitis and asthma display many similarities in their epithelial and inflammatory responses to allergens. However, one notable difference is that disruption and desquamation of the epithelium is a characteristic feature of asthma, whereas, in allergic rhinitis, the epithelium is intact. Airway remodeling is a well-recognized feature among patients with chronic asthma but not in allergic rhinitis. The purpose of this study is to investigate the airway remodeling in mice with experimentally induced airway allergy.
SUBJECTS AND METHOD
Male BALB/c mice were systemically sensitized with ovalbumin mixed with aluminum hydroxide gel solution and were challenged with aerosols of ovalbumin. Then, the mice were chronically exposed to aerosols of ovalbumin for 5 weeks to 10 weeks. After the last exposure, bronchoalveolar lavage fluid (BALF) and nasal lavage fluid (NLF) were collected. Next, the number of inflammatory cells and the concentrations of IL-5, IL-13, TGF-beta and IFN-gamma in BALF and NLF were measured. Finally, nasal cavity and lung specimen were obtained to evaluate the subepithelial fibrosis by trichrome stain and the expression of MMP-9 and TIMP-1 by immunohistochemical stain.
Results
The concentrations of IL-5, IL-13, TGF-beta within the BALF & NLF were found to have significantly increased in the 10-week exposed group compared with the control group. The expression of MMP-9, TIMP-1 and subepithelial fibrosis in the lung tissue were also found to have significantly increased in the 10-week exposed group comparative to that of the control group. But the expression of MMP-9, TIMP-1 and subepithelial fibrosis in the nasal mucosa were not prominent in the 10-week exposed group compared with the control group.
Conclusion
The results suggest that airway remodeling may be induced by repeated allergen exposure in allergic rhinitis but the structural changes of the nasal mucosa by remodeling process are less prominent compared with the lung tissue. |
| Key Words:
Allergic rhinitis;Airway;Remodeling |
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