Journal of Rhinology 2008;15(2):129-133.
Published online November 30, 2008.
Expression of TARC in Nasal Epithelial Cells by IL-4/IL-13 and TNF-alpha in Allergic Rhinitis
Kun Hee Lee, Sung Wan Kim, Seung Yup Shin, Joong Saeng Cho
Department of Otorhinolaryngology-Head and Neck Surgery, Kyung Hee University, School of Medicine, Seoul, Korea. minslee@khnmc.or.kr
알레르기비염환자 코점막 상피세포에서 IL-4, IL-13, TNF-α 자극에 의한 Thymus and Activation-Regulated Chemokine의 발현
이건희, 김성완, 신승엽, 조중생
경희대학교 의과대학 이비인후과학교실
Abstract
BACKGROUND: Thymus and Activation-Regulated Chemokine (TARC) is a highly specific ligand for CCR4 expressed in Th2 lymphocytes. Local production of TARC may play an important role in the induction and maintenance of allergic inflammation with the infiltration of Th2 lymphocytes. However, the cellular sources of TARC among patients with allergic rhinitis (AR) remain unclear. OBJECTIVE: We investigated that nasal epithelial cells from AR could produce TARC and that they could produce TARC differently by various stimulation of cytokines. METHODS: Inferior turbinate mucosal tissues were collected from six patients with AR sensitized to house dust mite. Nasal epithelial cells were isolated, cultured and stimulated with IL-4, IL-13 or TNF-a alone or in combination. The level of TARC in the supernatant was measured by ELISA and mRNA expression of that in the cells by RT-PCR. RESULTS: The level of TARC from cultured nasal epithelial cells (CNEC) among allergic rhinitis patients was higher than that in the control group. IL-4 or IL-13 or TNF-a alone did not upregulate TARC production from CNEC. However, Th2 cytokines in combination with TNF-a increased the production of TARC in CNEC. CONCLUSION: IL-4, IL-13 and TNF-a could upregulate TARC production from nasal epithelial cells in allergic rhinitis and contribute to the infiltration of Th2 cells to the tissue during allergic inflammation.
Key Words: TARC;Epithelial cells;Allergic rhinitis




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