| The Effect of Cigarette Smoking on the Expression of MMP-9, TIMP-1 and VEGF in Nasal Polyp Epithelial Cells and Fibroblasts |
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Yun Suk An, Jun Mo Kim, Jong Hwan Wang, Yong Ju Jang |
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Department of Otolaryngology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. jangyj@amc.seoul.kr |
| 흡연이 비용종 상피세포와 섬유아세포에서 MMP-9, TIMP-1, VEGF의 발현에 미치는 영향 |
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안윤숙, 김준모, 왕종환, 장용주 |
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울산대학교 의과대학 서울아산병원 이비인후과학교실 |
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| Abstract |
Background and Objectives
It has been known that MMP-9, TIMP-1, and VEGF play an important role in the formation of nasal polyps (NPs). The aim of this study is to investigate the effect of smoking on the expression of MMP-9, TIMP-1 and VEGF in the epithelial cells and fibroblasts of NPs.
MATERIALS AND METHODS
The epithelial cells and fibroblasts of NPs obtained from 10 patients who underwent endoscopic sinus surgery were cultured and used. The prepared CSE concentrations were 5%, 1%, 0.5% and 0.1%. In the control group, cells were cultured for 9 hours in the media containing 1 ml of AEGM and DMEM/F-12K. In the CSE group, cells were treated with 5%, 1%, 0.5% and 0.1% CSE for 9 hours. After washing with PBS, cells were cultured in medium for 24 hours. ELISA was performed to measure the secretion of MMP-9, TIMP-1 and VEGF proteins and real-time PCR was performed to assay their mRNAs.
Results
The production of MMP-9, TIMP-1 and VEGF proteins did not change significantly in comparison with the control group in epithelial cells and fibroblasts after exposure to CSE.
Conclusion
These results suggest that CSE does not appear to have an effect on the expression of MMP-9, TIMP-1 and VEGF for developing and growth of nasal polyposis. |
| Key Words:
Nasal polyp;Smoking;CSE;Epithelial cell;Fibroblasts |
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