Journal of Rhinology 2011;18(1):35-42.
Published online May 31, 2011.
Effects of Nasal Instillation of Staphylococcal Enterotoxin B on Nasal and Bronchial Mucosa in BALB/c Mouse
Soung Yong Jin, Eung Hyup Kim, Gun Ho Lee, Yeong Kyu Park, Yong Min Kim, Ki Sang Rha
1Department of Otorhinolaryngology-Head and Neck Surgery, Chungnam National University, School of Medicine, Daejeon, Korea.
2Research Institute for Medical Science, Chungnam National University, School of Medicine, Daejeon, Korea.
BALB/c 마우스에서 포도상구균 내독소의 비강내 점적이 비점막 및 기관지 점막에 미치는 영향
진성용, 김응협, 이건호, 박영규, 김용민, 나기상
충남대학교 의학전문대학원 이비인후과학교실,1 의학연구소2
Background and Objectives
The effect of nasal exposure to staphylococcal enterotoxin in the pathogenesis of allergic rhinitis remains controversial. We sought to determine the effect of increasing doses of intranasally applied Staphylococcus aureus enterotoxin B (SEB) on the respiratory mucosa, especially the nasal mucosa.
Materials and Method
S: Nasal application of SEB was performed on four occasions (days 0-4-8-12) in unsensitized BALB/c mice. Control mice were intranasally treated with phosphate buffered saline (PBS), and 5 ng, 50 ng, 500 ng, and 5 microg of SEB was applied to the respective experimental group. The concentrations of IL-4, IL-5, and IFN-gamma in bronchoalveolar lavage fluid (BALF), nasal lavage fluid (NLF) and serum were compared among groups. Also, the counts of total inflammatory cells, macrophages, lymphocytes, neutrophils, eosinophils, and basophils in BALF and NLF were compared among the groups. Pathologic studies for inflammatory cell infiltration in the nasal mucosa and peribronchial area were conducted.
IL-4 and IFN-gamma showed higher concentrations with increasing stimulation dose of SEB in NLF and serum. The IL-5 concentration showed a tendency to increase in NLF and serum, but these changes were not statistically significant. Total inflammatory cell count, especially macrophage count, in BALF and NLF was higher with increasing stimulation dose of SEB. Infiltration of inflammatory cells into the nasal mucosa showed a tendency to increase in a dose-dependent manner.
These results suggest that nasal exposure to SEB may induce Th1 and Th2 inflammatory responses in the respiratory mucosa, especially the nasal mucosa.
Key Words: Allergic rhinitis;Enterotoxin;SEB;Lavage fluid

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